Kuze kube manje, indlela ye-PCR (i-polymerase chain response) ingenye yezindlela ezifundisa kakhulu futhi ezinembile kakhulu zokunquma ukutheleleka emzimbeni womuntu. Uma kuqhathaniswa nezinye ukuhlaziywa, akanayo umkhawulo wokuzwela, okuvumela ukubona i-DNA ye-agent esebenzayo kanye nemvelo yayo.
I-PCR yisimiso sendlela
Okuyinhloko yendlela ukucacisa futhi kwandiswe ngokuphindaphindiwe ingxenye ye-DNA ye-pathogen emthonjeni wezinto eziphilayo ezitholakalayo esifundweni. Ukuphuma, ukuxilongwa kwamangqamuzana ngendlela ye-PCR, ungakwazi kalula ukuthola noma iyiphi i-DNA ne-RNA yezincane ezincane. Njengoba ngamunye wabo enezakhi zofuzo eziyingqayizivele, okuyilapho, uma i-fragment efana nayo itholakala kwisampula yezinto eziphilayo, iqala inqubo yokwakha inani elikhulu lamakhophi. Ngokuqondene nalokhu, ukucaciswa kwalendlela kuqinisekisa umphumela onembile, ngisho noma kuphela i-DNA ingxenyana yesifo esitholakala kwisampula.
Ngaphezu kwalokho, ukuxilongwa kwamangqamuzana esebenzisa indlela ye-PCR kanye nokuchitshiyelwa kwayo okulandelayo kuhilela ukutholakala kwe-ejenti esithathelwanayo ngisho nesikhathi sokutshala, lapho ukubonakaliswa kwemitholampilo yesifo engekho.
Isimo esibaluleke kakhulu sokuqhuba i-PCR ukulungiselela kwangaphambili kanye nesampula efanelekile kwento.
Indlela ye-PCR - kuthathwa kanjani?
Enye yezinzuzo eziphawulekayo zendlela ukuthi izinto eziphilayo ezihlukile ngokuphelele zifanelekile ukutadisha. Kungaba uketshezi lwesisu , ama-smears avela emlonyeni wesibeletho noma urethra, umchamo noma igazi. Konke kuncike ekutheni i-pathogen ne-habitat yayo.
Ngokujwayelekile, ukuthola ukuthi izifo ezithathelwana ngokobulili zisebenzisa indlela ye-PCR, imfihlo yokuhlukumeza ngokocansi ithathwa ukuthola ukuthi i-virus yesifo sofuba noma i-HIV ithathwa yisampuli segazi.
Kunoma yikuphi, odokotela batusa ngaphambi kokunikeza ukuhlaziywa:
- Izinsuku ezingu-1-3 ngaphambi kosuku olulindelekile lokuqoqa izinto zokwakha ukugwema ubulili;
- Ngaphambi nje kokuzinikela, akukho lutho ekuseni;
- kusihlwa, hhayi ukugeza nge- sopha antibacterial , kodwa ekuseni nje ngamanzi, hhayi douche;
- Amahora angu-2-3 ngaphambi kokuvivinywa, ungagcini;
- kungcono ukuqhuba ukuhlolwa ngokushesha emva kokuphela kokuya esikhathini.
Kusobala ukuthi i-PCR yindlela yokucwaninga nephezulu yezobuchwepheshe, okulula ukuyisebenzisa, futhi inezinkomba zokunakekelwa okukhulu. Ngaphandle kwemithi ewusizo, i-polymerase chain reaction isetshenziselwa izinhloso zesayensi.