Le ndlela, naphezu kokusakazeka kwayo, inezinkinga ezithile. Yilokho okwenza ochwepheshe bafune izindlela ezintsha ezivumela ukugcina i-ejaculate isikhathi eside. Ukungalungi okuyinhloko kwendlela yokwelapha i-cryopreservation okukhulunywe ngenhla kungabizwa yiqiniso lokuthi emva kokuqeda ngokuphelele isidoda, ukuhamba kwamaseli wesondo okuqukethwe kuwo kunciphisa cishe ngo-20-25%. Lokhu kusho ukuthi amathuba okukhulelwa ngesikhathi sokukhulelwa kweqanda elivuthiwe elinjalo le-spermatozoa nalo liyancipha.
Lapho ukwelapha umuthi ngale ndlela, ukugcina imbewu uthola izinga lokushisa kwama-degree angu -196.
Indlela yokugcina isilwane sobuchwepheshe K. Saito
Le ndlela yokulondoloza u-ejaculate wesilisa ingasetshenziswa kulawo maso uma kungekho sidingo sokulinda isikhathi eside i-IVF. Akubandakanyi ukunqanda kwesidoda.
Uma usebenzisa le ndlela, izimo zokugcina zesithombo zithatha ukusebenzisa okuthiwa yi-electrolyte-free medium (BES). Ngakho-ke, isisombululo esingabawoti, isixazululo se-aqueous of glucose sisetshenziswa njalo. Kumele uqaphele ukuthi kuze kube sekupheleni komgudu wokulondoloza ukutholakala kwamaseli wesilisa ocansini kuleso sixazululo akuzange kufundwe. Kodwa-ke, ucwaningo oluningi luye lwabonisa ukuthi ama-sodium ne-potassium cations avinjelwe emakhazeni, okungafakwa ngaphandle uma isotonic glucose isetshenziswa. Ngamazwi alula, ukusebenzisa lezi zixazululo zokugcina u-ejaculate wesilisa kuvumela ukulondoloza i-spermatozoa ngaphandle kweqhwa, ngaphandle kokushintsha izici zabo zokuziphatha.
Le nqubo ayivumeli ukugcina isilwane isikhathi eside nje ngenkathi i-cryopreservation. Yingakho ingasetshenziswa, isibonelo, uma uthatha ama-biomaterials izinsuku ezimbalwa ngaphambi kwe-IVF noma lapho ukukhulelwa okulandelayo kudingekile uma lowo owedlule wehlulekile.
Ingakanani imbewu engagcinwa?
Lolu hlobo lombuzo luyisisekelo kakhulu kulawo madoda angakalungi kulo mzuzu ukuba abe obaba.
Okokuqala, kufanele kuqashelwe ukuthi konke kuxhomeke ekukhethweni kwendlela yokugcina i-ejaculate yesilisa. Cishe zonke izinhlobo zamabhange zamanje zisebenzisa indlela yokuguqulwa kwe-cryopreservation. Ikuvumela ukuba ugcine i-ejaculate isikhathi eside - kuze kube amashumi eminyaka ambalwa.
Uma usebenzisa inqubo engabandakanyi ukukhishwa kwe-ejaculate, ingagcinwa ngaphezu kwenyanga eyodwa. Njengoba sekushiwo ngenhla, le ndlela isetshenziselwa ikakhulukazi inqubo ye- in vitro fertilization.
Kodwa-ke, isikhathi esincane esifanele sokulondoloza isisu asithinteki i-motility ye-spermatozoa.
I-ejaculate igcinwe kanjani?
Yona kanye inqubo yokukhethwa kwesilisa iqhutshwa esikhungweni sezokwelapha esikhethekile. Indoda inikezwa i-flask oyinyumba, lapho i-ejaculate iqoqwa khona ngokushaya indlwabu.
Ngemva kwalokho, i-flask ngokwayo ifakwe kudivayisi ekhethekile, i-reagent lapho kuvela khona i-nitrogen enamandla, futhi ivaliwe ngokuqinile.
Uma kunesidingo, isibhamu sesikhumba sisusiwe futhi sichithwa. Khona-ke, ikhwalithi yokulondolozwa kwayo ihlolwe ngokuhlola isampula ku-microscope ekhethekile.