Ukwenziwa kwe-cryopreservation yemibungu kuyindlela evumela ukuba bahlale bephila iminyaka eminingana nekhono elondolozwe lokuhlukanisa ngemuva kokudonsa. Inqubo ye- cryopreservation yenziwa ngokuphatha ama-embryo nge-nitrogen ene-liquid. Kulesi sihloko sizocabangela izici zenqubo yokuqhwaza, izinhlobo zabo, kanye nokucaca kwemibungu e-cryoprotective emgodini we-uterine ngesikhathi sokukhulelwa kwe-in vitro .
Ubuchwepheshe bombungu buqhwa
Kunezindlela ezimbili zemibungu yokuqandisa: i-vitrification kanye "nokukhanda kancane". Ubuchwepheshe bokuthi "ukukhanda kancane" kubhekwa kungasebenzi futhi sekuyeke ukusetshenziselwa emitholampilo eminingi. Iqukethe ukuhambisa ama-embryo acwebekile abe ngotshani nge-cryoprotectant, bese kukhishwa amanzi okuqandisa. Ukuqhaqhaza umbungu kuwuvikeleka eqhweni, kepha kungaholela ekudambiseni amanzi. Lokhu kunciphisa inani lemibungu engakwazi ukuphela kuphela, kepha futhi nokugcina ikhono lokuhlukanisa nokuhlukanisa.
I-vitrification yemibungu iyindlela ejwayelekile kakhulu, ehlanganisa ukuwaphatha nge-nitrogen eyetshezi, kuyilapho amanzi abaqukethe aguqulwa abe yi-jelly.
Imishini ye-cryopreservation iyabiza kakhulu, ikakhulukazi eyasetshenziselwa "ukukhanda kancane". Ishalofu-ukuphila kwemibungu eqinile akufanele kudlule iminyaka emihlanu.
Ukudluliselwa kwemibungu emva kokukhishwa kwe-cryopreservation
I-embryo, ephuma emakhazeni ngaphambi kokuba ifakwe esibelethweni, kufanele ilungiselelwe. Ukuze benze lokhu, bahlushwa ekamelweni lokushisa futhi baphathwa ngesisombululo esikhethekile esizosiza umbungu ukuba ugcine izakhiwo zawo. Ukudluliswa kwemibungu ekhishwe ngamathambo kwenziwa nge-catheter ekhethekile, efakwe emgqonyeni wesibeletho emgodini we-uterine.
Sasihlola ubuchwepheshe obukhona be-embrying ekhanda, kanye nenqubo yokuphindaphinda imibungu efriziwe. Ukwelashwa kwama-embryo kwenza kube lula ukufaka kabusha uma kungekho ukukhulelwa emva kwenqubo yokuqala.